The standard diagnostic test for chronic cystitis have been ineffective
Urinary tract infections (cystitis and pyelonephritis) are of great prevalence, but their accurate diagnosis is often difficult. The authors of the new study argue that the standard methods for the detection of bacteria in urine does not give accurate results in most chronic patients.
Each year urinary tract infections (UTI) faces about 150-200 million people worldwide. Many people, especially young women, suffer from bouts of acute cystitis, which quickly pass after the use of antibiotics, and with chronic infection people can struggle for many years.
From chronic infections suffer more older women, but they have a bacteriological test for UTI is often negative. Analysis, which applies the 1950-ies around the world, inefficient, said Dr. Jennifer Ron (Jennifer Rohn) from University College London (University College London).
A group of researchers under her leadership, compared the results of the standard test bacterial culture from the middle portion of the urine with the test DNA sequencing in 33 patients with symptoms of UTI (30 of them relapsed after treatment) and in 29 patients in the control group without these symptoms. As a result, only six patients the cultures were positive, while the DNA test identified the infection in all patients of study groups. These data were published by the Journal of Clinical Microbiology.
Our study compared conventional bacterial analysis with modern methods of DNA analysis, and showed that the old test is not effective. On the other hand, genome sequencing with the use of enriched urine test easily allowed to identify the really sick people, says Ron.
According to Ron, in relation to the urine has long been bad suspicions, but these suspicions were validated in the study.
According to her, UTI is frequently faced is not just young women, which they held shortly after treatment with antibiotics, but many of the older people, patients with spinal cord injuries, multiple sclerosis, kidney transplant, pregnant women.
The inefficiency of this analysis leads to the fact that many people who need antibiotic treatment, denied treatment on the basis of the results of the analysis. As a result, many are forced to quit work or study. Many have to refuse sex because it causes pain. There are cases when women end up in the hospital with sepsis as the result of undiagnosed UTI.